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Product Info Summary
| SKU: | A09539 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-AP-3 complex subunit beta-2 AP3B2 Antibody
SKU/Catalog Number
A09539
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AP-3 complex subunit beta-2 AP3B2 Antibody (Catalog # A09539). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AP3B2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AP-3 complex subunit beta-2 AP3B2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09539)
Host
Rabbit
Contents
AP3B2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
AP3B2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2. The immunogen is located within the first 50 amino acids of AP3B2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.
Reactive Species
A09539 is reactive to AP3B2 in Human, Mouse, Rat
Applications
A09539 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
119.059kDa
Background of AP3B2
The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AP3B2 in rat brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of AP3B2 in mouse brain tissue with AP3B2 antibody at 5 μg/mL.
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Immunofluorescence of AP3B2 in mouse brain tissue with AP3B2 antibody at 20 μg/mL.
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Western blot analysis of AP3B2 expression in mouse brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For AP3B2 (Source: Uniprot.org, NCBI)
Gene Name
AP3B2
Full Name
AP-3 complex subunit beta-2
Weight
119.059kDa
Superfamily
adaptor complexes large subunit family
Alternative Names
Adapter-related protein complex 3 subunit beta-2; Adaptor protein complex AP-3 subunit beta-2; adaptor-related protein complex 3, beta 2 subunit; beta-3B-adaptin; Clathrin assembly protein complex 3 beta-2 large chain; DKFZp686D17136; NAPTBAP-3 complex subunit beta-2; Neuronal adaptin-like protein, beta-subunit; Neuron-specific vesicle coat protein beta-NAP AP3B2 DEE48, EIEE48, NAPTB adaptor related protein complex 3 subunit beta 2 AP-3 complex subunit beta-2|Neuronal adaptin-like protein, beta-subunit|adaptor protein complex AP-3 subunit beta-2|adaptor related protein complex 3 beta 2 subunit|beta-3B-adaptin|clathrin assembly protein complex 3 beta-2 large chain|neuron-specific vesicle coat protein beta-NAP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP3B2, check out the AP3B2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP3B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-3 complex subunit beta-2 AP3B2 Antibody (A09539)
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